Expression analysis and ChIP experiments were performed and analysed as described previously (Krejcí et al, 2009) with the following modifications: (i) For each biological replicate RNA obtained from 60 wing discs was reverse transcribed and hybridized on long-oligonucleotide FL003 INDAC micro-arrays representing 14 444 transcripts from release 5 of the Drosophila genome.
Additionally, if such support be necessary, it should help to listen to the disc in exact reverse order, starting with the arrangement of "Ich bin der Welt abhanden gekommen", which superadds otherworldliness to Mahler's already ethereal beauty.
Therefore, inhibiting IL-1 could be an important therapeutic target for preventing and reversing disc degeneration.
Several studies have suggested the use of anabolic growth factors to regenerate the matrix of the IVD and hence restore disc height, thereby reversing degenerative disc disease.
The rabbit models suggest that prolonged disc distraction might reverse some aspects of the compression-induced degeneration with better results at the L3/L4 level [ 47– 51].
Attenuating the catabolic effects of IL-1β in the NP may be an essential therapeutic step in slowing or reversing disc degeneration.
As no effective therapies to retard or reverse disc degeneration have yet been devised, there is huge interest in potential regenerative treatments for human patients.
This study has shown how IL-1, a naturally occurring cytokine within the IVD, could, through an imbalance between it and its inhibitor, play a role in the pathogenesis of IVD degeneration and therefore be an important therapeutic target for preventing and reversing disc degeneration.
The use of anabolic growth factors as therapies to regenerate IVD matrix, hence restoring disc height and thus reversing degenerative disc disease, has been suggested.
Cervical cages relieve neuraxis compression through the restoration of disc space height, thereby reversing the loss of foraminal height and area, and cervical canal diameter [ 49, 55, 56].
