In this manuscript, we showed that the HD-only form of Ey can promote cell growth and proliferation during imaginal disc development.
Leg disc development is, however, very different from eye development and the developmental consequences are therefore distinct.
Previous studies have shown that inhibition of wg signaling during wing disc development results in adult wing margin notches of varying severities [14].
Other highly significant GO categories are "Imaginal disc development", "Instar larval/pupal development", and "Metamorphosis", consistent with the source of the tissues used for these experiments.
This striking enrichment clearly demonstrates that the set of Ubx-bound genes are functionally important in aspects of imaginal disc development.
In wing disc development, the Wingless (Wg) member of the Wnt family organizes the dorsal/ventral (D/V) axis, while BMPs are is required to pattern the anterior/posterior (A/P) axis (reviewed in [13]).
In an effort to monitor changes in the subcellular localisation of Armadillo during third instar larval wing imaginal disc development, we used a stock expressing an Armadillo-GFP construct under the Armadillo promoter.
Overall, the consistency between these GO categories and the expected functions of Ubx in imaginal disc development suggests that many of the Ubx target genes identified by these ChIP experiments are biologically relevant targets.
Two additional noteworthy categories are "Wing disc development" and "Wing disc pattern formation", both of which are more significantly associated with the haltere data sets compared to the leg data sets.
This experiment was predicated on previous studies reporting strong genetic interactions between Rho1 and several components in the Rho signaling pathway, including RhoGEF2 and zip, during leg imaginal disc development [23] [26].
GO analysis of the target genes for these high-confidence peaks revealed significant enrichment for developmental processes such as imaginal disc development (Supplementary Tables S5 and S6), again suggesting that these are biologically important binding events.
