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Tumor and normal tissues were processed by using the same methodology, based on mechanical disaggregation of tissue followed by enzymatic digestion and cell purification by sequential sieving.
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It is based on mechanical disaggregation of the tissue followed by enzymatic digestion and cell purification by sequential sieving.
The enzymes generally included are collagenase and mainly trypsin which, even though they contribute to an adequate disaggregation of the tissue [ 3], they can also affect cell integrity and/or preservation of macromolecules of interest.
In contrast, conditionally targeted knockout of ilk in the mouse heart causes left ventricle dilation, heart failure, disaggregation of cardiac tissue, leading to sudden death (White et al., 2006).
Lactate creates a microenvironment of low pH around the embryo to assist the disaggregation of uterine tissues to facilitate trophoblast invasion.
After disaggregation, the pieces of tissue were washed three more times in PBS, this time with centrifugation.
After mechanical disaggregation of the tumour tissue into 1 2 mm pieces, the minced tissue was enzymatically digested with 2 mg/ml collagenase B (Roche Diagnostics, Mannheim, Germany) for approximately 20 hours under constant rotation at 37°C.
Improved detection of diffuse large B-cell lymphoma by flow cytometric immunophenotyping-Effect of tissue disaggregation method.
Chen S, Wetzel R. Solubilization and disaggregation of polyglutamine peptides.
Disaggregation of other knowledge products would complement our work.
The disaggregation of a protein complex into its constituent components.
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