Attached cells were removed and disaggregated from each sample surface by vortexing for 30 seconds, sonicating for 2 minutes in a bath sonicator (Branson 3510) and vortexing an additional 30 seconds [34].
Streamflow is disaggregated into a subsurface component and a surface component that, in turn, is considered formed by two different contributions: impervious runoff and surface runoff from permeable areas induced by heavy rains.
Alternatively, it has been suggested that cellulose deconstruction is a two-step process where the cellulose-rich fibers are initially fragmented or disaggregated into shorter fibers, resulting in a greater overall surface area for the enzymes to subsequently attack [ 9- 12].
Because maize cell walls used in our lignification experiments were fully disaggregated and extremely thin (~1 μm), they exposed a large surface area that could be quickly colonized by rumen microflora [ 24].
Briefly, tumours were excised at 3 days after vaccination, weighed, enzymatically disaggregated, and the obtained single cell suspensions counted and subjected to surface staining with antibodies for three-colour flow cytometry.
Lumped values of near-surface and deep soil water content are then disaggregated at local scale using simple relationship between mean quantities, local topography and soil depth information.
This paper demonstrates the ability to retrieve soil hydraulic parameters from near-surface measurements, using Soil Moisture and Ocean Salinity (SMOS) observations disaggregated to 1 km resolution for a demonstration area the size of a single SMOS footprint.
However, this is a reversible state because adding the disaggregating drug dissolved a portion of the staples and allowed disaggregated CD8lumenal to behave as a proper anterograde cargo that is glycosylated in the Golgi prior to being targeted to the cell surface membrane.
Companies are getting smaller and businesses are being disaggregated.
