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The protein contained a domain that is designed to self-aggregate, but which can be triggered to disaggregate in the presence of a small molecule (Rollins et al., 2000).
TAM purification procedure was derived and improved from Sierra et al. In brief, B16 melanoma tumors grown in C57BL/6 mice were surgically removed, minced, and left to disaggregate in 0.1% collagenase I-RPMI-1640 I-RPMI-1640 I-RPMI-1640solution at 37 °C for 60 min.
Taking into account that dead planarians disaggregate in the medium in few hours and that extracted RPM sample planarians still conserved a recognizable structure (see Figure 2(b)), we conjectured that simulated microgravity did not cause the immediate death of planarians but they rather died close before the end of the simulation.
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After 2 days, the cells were centrifuged at room temperature for 5 min at 100 g and mechanically disaggregated in proliferation medium to obtain individual cells (Fig. 3A2, disaggregation).
For Sundararajan, the sharing economy provides a way for real-world assets to be disaggregated in space and time and repackaged into standalone services.
For a structured overview, the EECA region is disaggregated in three subregions, namely, (a) Russian Federation, (b) Eastern European countries (without Russia) and (c) Central Asian countries.
Synergy at the national level can then be disaggregated in terms of geographical levels (regions, provinces) or in the other two dimensions (sectors and size classes).
Different constitutive models are found to produce a good simulation of experimental moment curvature behaviour, depending on whether experimental data are disaggregated in terms of masonry type or not.
Table 1 shows the absolute and the relative numbers of all occupied persons in Spain, disaggregated in four occupational groups: Employees (excluding managers), Employers, Managers, and Own-Account Self-Employed.
The samples were frozen after collection and later thawed, dried at 50 60 °C in an oven and disaggregated in an agate mortar, before chemical treatment for total metal analysis.
The recommendations are disaggregated in five major topics, which are reflected in the context of S&T internationalisation objectives as outlined in the 'Internationalisation of R&D from an S&T policy perspective' section of this article, as follows.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com