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Disadvantages to the system include the need for sufficient experimental replication of transcription assays to compensate for microinjection damage or frog-to-frog variation in oocyte quality.
Disadvantages to the system indicated by the HSAs included cost (49%%; 21/43, 2 missing) and the unavailability of phones (51%%; 22/43, 2 missing).
Almost one third of respondents (9/29) indicated that they could not identify any disadvantages to the system, and 21%% (6/29) indicating that charging their phone was a problem.
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An obvious disadvantage to the system is that the genetic linkage between the gene of interest and the XFP transgene is in most cases incomplete.
The major disadvantages to these systems are that the gelation rate is hard to control; the resulting structure is not uniform; and mechanically strong and complex-shaped 3-D structures are difficult to achieve.
The disadvantage to this system is that the furnace is sized to the house and has limited ability to raise the temperature.
However various studies have also brought to light certain disadvantages to these systems.
However, as with all protein expression systems, there are advantages and disadvantages to these systems which are described in several review articles [ 1– 3].
The disadvantage to this system is that instruction tends to be boring and repetitive, with tests and questions following the same pattern for every course.
Another disadvantage to the forced-air system is that the air handler and blower unit can be noisy, especially with older units.
However, the submission admits there are considerable disadvantages to a system of complete deregulation.
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