The main disadvantage of expressing a recombinant protein in inclusion bodies is the high operational cost for its recovery in a soluble way when it is needed [ 33, 40].
To test advantages and disadvantages of expressing each channel in hippocampal neurons, we used sparse transfection of neurons to avoid complications and confounds of widespread neuronal activation and associated release of neurotransmitters and modulators.
The need for optimizing MGMT protein design is supported by recent observations suggesting that the P140K mutant of MGMT, developed to be resistant to inhibitors such as O6-benzylguanine, may confer a selective disadvantage when expressed at high levels.
It is worrying that even in a country as Norway, with one of the highest human development indices in the world, disadvantage as expressed by low education of parents and parental disability is still handed down from generation to generation.
We demonstrate that Bcl-x is non-essential for RIP1-Tag2 tumorigenesis, and that cells harboring null alleles of Bcl-x do not have an inherent survival disadvantage to cells expressing wild type Bcl-x.
Again, we used the ΔNGFR surface marker to distinguish the transduced population from the untransduced in order to take into account the growth disadvantage of cells expressing p53-activating peptides.
In spleen cells of 3 week old mice, normal and mutant dyskerin proteins were present in roughly equal proportions, but by 22 weeks the mutant dyskerin represented only 16% of the total pool, suggesting a growth disadvantage for the cells expressing the mutant protein.
Not having seen Moore's latest movie, "Sicko," DiLorenzo was at something of a disadvantage, but he expressed outrage at Moore's failure, in his previous films, to recognize the importance of competition, the virtues of sweatshops, and the depredations of socialism.
Gender disadvantage is often expressed through domestic violence towards women.
Presumably, stabilizing selection optimizes copy number at the Hsp70 locus in D. melanogaster, balancing the advantage of additional copies that enable a more rapid or robust response to environmental stress with the disadvantage of physiological costs of expressing additional copies.
