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Pristine graphene was directly suspended in a variety of organic media, which are used either as electrolyte solvents or deposition media in sensitized solar cells.
Some functional equipment, such as traction transformer, traction converter, and waste collection unit are directly suspended under the carbody chassis; some of them weigh tons and bear vibration excitation sources such as the cooling fan and mechanical switch.
Different operational modes have developed from LPME; the most commonly used of which are as follows: single-drop microextraction (SDME) [22], headspace solvent microextraction [11], hollow fiber-based LPME (HF-LPME) [23, 24], directly suspended droplet microextraction [25], ionic liquid-based single-drop microextraction [26], and dispersive liquid-liquid microextraction [27].
For the AS02A formulation, the lyophilized antigen was directly suspended in 0.5 ml of the adjuvant.
For Figure 5A, 10 to 15×106 cells of CCRF-CEM cells were directly suspended in 10% serum containing RPMI without serum starvation, then treated with or without taxol and nocodazole as described above and used for RhoA pulldown assay.
DNA fragments in the aqueous phase were precipitated by adding 10 µL ammonium acetate (3 M), 2 µL glycogen (10 mg/mL) and 2.5 vol ethanol, washed with 75% ethanol, dried and directly suspended in 10 ml of 90% formamide-loading gel buffer (10 mM Tris-HCl, pH 8.0, 20 mM EDTA, pH 8.0, 0.05% bromophenol blue, 0.05% xylene cyanol).
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For the regular suspension, a formulation was made by directly suspending bulk DCU in the vehicle.
The formulation was made by directly suspending the bulk drug in 0.5% (w/w) Tween 80 in PBS.
After removal of the white blood cells (WBC), an aliquot (~100 μl) of the erythrocyte portion was directly re-suspended in TRIzol and kept at −80 degrees till use.
IP was done using 9E10 and 9E11 (anti-Myc) antibody, or mouse IgG control antibody (Sigma-Aldrich), chemically crosslinked to Protein A/G Agarose beads (Lane and Harlow, 1988 ), and the IPs washed extensively before being directly re-suspended in SDS-loading buffer and subjected to western blotting.
Don't dig straight down, as the End Portal frame is suspended directly over a shallow lava pool!
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