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And finally the design vectors are directly sorted according to the DICV based preferential guidelines.
Viable CD8+ T-cell subsets were directly sorted and aliquots spun down and dry stored at −80ºC until DNA isolation.
This may be concluded from data of the Fujiki laboratory (Matsuzaki and Fujiki 2008), who demonstrated that in mammals Pex3 is directly sorted to peroxisomes.
We propose a robust and reproducible procedure, which includes whole transcriptome gene expression profiling of major subsets of immune cell cells directly sorted from whole blood.
For cell sorting a total number of 5 × 106 cells per replicate were directly sorted on a filter well plate (LoProdyne™ membrane with 0.45 μm pore size, Nunc, Germany) and the residual buffer was constantly drawn off by an exhaust pump.
Single MDDCs were directly sorted into 384-well PCR plates as previously described [14].
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Several kinases that also inhibited the tumor-initiating CD44high population in SUM149 after siRNA treatments were identified and tested directly against sorted CD44high/CD24-/low CD44high/CD24-/low
NBs were directly FACS-sorted onto the petri dish, and also left to settle for 2 hr before live imaging.
Nevertheless, we should note that proposition 1 is still slightly different from the work of Ling and Mow [39] in two senses: (a) while Ling and Mow [39] directly implemented the sorted QR technique to re-arrange the basis vectors, we do not assume any sorting in proposition 1 and (b) as a result of (a), the proofs given here and in Ling and Mow [39] are essentially different.
This led to the test of these 12 kinases directly against a sorted CD44high/CD24-/low CD44high/CD24-/lowUM149.
Further tests of these 12 kinases directly on a sorted CD44high/CD24-/low CD44high/CD24-/lowof SUM149 cells confirmed TICir effect.
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