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The ligation reactions with average insert size of 100 kb or more were mass-transformed, plated, and directly picked with toothpicks into 384-well plates.
In 2004 Yudhoyono, or SBY as he is known, won the country's first election in which voters directly picked the president.
Silver staining can be used to visualize protein features on a gel to facilitate excision of the relevant spots for MS. Alternatively, spots are directly picked from fluorescent gels using a robotic spot picker.
Individual glands on the bottom of the dishes were directly picked up one by one under a microscope, collected into Eppendorf tubes and seeded onto 24-well dishes for subsequent gene transfection by viral vectors.
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A ghost signal caused by the receiver directly picking up the transmitted signal is also present on Channel 1.
"Washington should find ways to reduce tensions by engaging Iran directly, picking up where the nuclear deal left off," wrote Nasr.
Here is something – his desire to reform the defence budget so that they are not paying for Cold War-era weapons systems that aren't used – we can directly pick up on by abandoning Trident.
Many are also excited about working in a system with a new leadership structure that will give the mayor control of the Board of Education and allow him to directly pick the chancellor.
Some advisors directly pick stocks, while others pick mutual funds (often called wrap accounts or fund of funds).
While MissionU will expand over time and looks to grow beyond San Francisco next year, that kind of focus might end up helping students that would otherwise have a difficult time directly picking a major.
Local organizing should focus on finding ways to directly pick up the slack for the federal government's dereliction of duty.
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