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Firstly, tumour samples were directly labelled with Spectrum Red and hybridised with biotin labelled reference DNA.
Ten additional isopods were directly labelled with eicosane-d42 as described above (labelled isopods) and extracted to verify that the isopod cuticle is able to adsorb the labelled compound.
Values in the same order have previously been reported for directly labelled 125I antibody conjugates [39, 48].
Additionally, bladder uptake is consistent with previous studies of directly labelled antibodies and is thought to result from transchelation of 99mTc from disulphides to endogenous cysteine [31].
Fresh human uterine and subcutaneous gluteal fat tissues were directly labelled using fluorescently conjugated Ulex Europaeus Agglutinin I (UEA-1) to visualize the three-dimensional nature of the vascular network.
However, in vitro experiments performed by Zhu et al. (2016) with lipid vessels of about 100 nm size, loaded with 225Ac and targeted with the antibody J591 against PSMA on human endothelial cells (HUVEC), could achieve 3 times higher cell killing efficacy compared with the same amount of 225Ac activity directly labelled to J591.
Most of these had all blastomeres directly labelled with DiI, as did additional non-dissociated specimens.
HAMLET protein was directly labelled with Alexa Fluor™ 488 (Molecular Probes Inc) according to manufacturer's instructions.
To prevent excessive chromosome denaturation, slides were placed on a slide warmer at 50°C for 1 hour. 1 µl of each of the X (directly labelled with FITC) and Y (directly labelled with CY3) probes (Cambio Ltd).
The staining order was: mouse anti-CD169, then goat anti-mouse, and finally the directly labelled antibodies, with washing steps in between to minimize cross-reactivity.
For directly labelled antibodies, cells were suspended in 100 µl of staining buffer and 10 µl of fluorochrome-labelled antibody added into the suspension.
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