Recovered FFPE tissue surrogate samples were analyzed by reversed-phase liquid chromatography (RPLC) coupled directly in-line with an Agilent 6340 ion trap mass spectrometer (Palo Alto, CA, USA).
Following a previous procedure [ 119] with a slight modification, 2-D LC ESI/MS/MS was performed using ProteomeX Workstation (ThermoFinnigan, San Jose, CA) that included the Surveyor auto sampler and the Surveyor HPLC unit coupled directly in-line with LCQ Deca XP Plus - Electro Spray Ionization (ESI) ion trap mass spectrometer capable of MS/MS (in time) analysis.
Peptides were separated by strong cation exchange (SCX) liquid chromatography (LC) followed by reverse phase (RP) LC coupled directly in line with electrospray ionization (ESI) tandem mass spectrometry (MS/MS).
LC analysis was accomplished by SCX followed by RP LC coupled directly in line with ESI IT MS. Samples were loaded into a LC gradient ion exchange system containing a Thermo Separations P4000 quaternary gradient pump (ThermoElectron) coupled with a 0.32×100 mm BioBasic SCX column.
Two-dimensional liquid chromatography (LC) analysis was accomplished by strong cation exchange (SCX) followed by reverse phase (RP) coupled directly in line with an electrospray ionization (ESI) ion trap tandem mass spectrometer (LCQ; ThermoElectron Corp., San Jose, Calif, USA) essentially as described in [19].
LC analysis was accomplished by strong cation exchange SCX) followed by reverse phase liquid chromatography (RP-LC) coupled directly in line with an ESI ion trap mass spectrometer (LCQ Deca XP Plus ThermoElectron Corporationn; San Jose, CA).
Briefly, protein mixtures are trypsin digested and the peptides desalted prior to strong cation exchange followed by reverse phase liquid chromatography coupled directly in line with ESI ion trap MS. A flow rate of 3 μL/min was used for both SCX and RP columns.
It is also observed that, the electrochemical bonds are formed between CNT and ZnO compounds and ZnO is directly coupled with C in the continuous chain of CNT lattice.
Various thioesters 137 were reacted with phenyldichlorophosphate to give the corresponding SATE derivatives 138, which were directly coupled with AZT in the presence of NMI.
In concordance with the previous studies [ 8, 18, 37], the correlation in ML condition in Sc suggested that antisense transcription is not directly coupled with transcription activity of the sense strand.
