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However, the paucity of efficient transformation methods and effective molecular tools for gene inactivation severely limits directly functional identification in L. bulgaricus CAUH1.
Resting-state functional magnetic resonance imaging (fMRI) approaches, which reveal patterns of coherent spontaneous fluctuations in the fMRI signal, offer a means to quantify directly functional interactions between the hemispheres.
In order to get at what makes Kate and Alex run — to get at their aesthetic sense and their personal commitment, or lack of it, to the work they do, to the meaning of their connoisseurship and its integration into their lives and their identities — Holofcener would need to open the movie up to more talk, talk that is, in a way, not directly functional.
In sequencing studies, on the other hand, the observed genetic variants are usually hypothesized to be directly functional and are of primary interest.
As rs78378222 changes the polyadenylation signal of TP53 leading to impaired 3′-end processing of TP53 mRNA, the SNP has strong plausibility for being directly functional contributing to the aetiological basis of glioma.
Although these data do not support rs2736100 being directly functional, in an analysis of putative binding sites at rs2736100 using TFSearch, SRY and Hfh-2 sites are only predicted for rs2736100-T and not rs2736100-G.
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To directly measure functional roles of uncultivated microbes, we developed Chip-stable isotope probing (SIP), a high-sensitivity, high-throughput SIP method performed on a phylogenetic microarray (chip).
Recently, we have identified a pool of transcription factors that are sufficient to convert skin fibroblasts directly into functional neuronal cells that we termed induced neuronal (iN) cells.
On the basis of this conclusion we previously identified transcription factor combinations that directly induce functional neuronal cells from mesodermal and endodermal cells.
The use of GFP fusions as a proxy for protein expression is an established approach but should be considered a starting point beyond which the protein of interest needs to be evaluated directly using functional assays.
Photodegradation and benzophenone-mediated crosslinking were observed both directly via functional group spectroscopies and indirectly via their effects on thin film surface properties and morphologies.
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