Sentence examples for directly frozen from inspiring English sources

The collected specimen was delivered on ice to the Tissue Bank, where it was divided into three parts: one in OCT embedding medium for frozen tissue specimens (Tissue-Tekâ) and two directly frozen in liquid nitrogen.

All samples were directly frozen in liquid nitrogen and stored at −70 °C.

To make the final product, cell pastes were directly frozen once with liquid nitrogen, thawed, frozen again and dried with a lyophilizer to obtain a dry cell powder.

The collected root tissue for Pythium quantification, consisting of six roots, was split in two biological replicates (except at time points 2, 10 & 20 DAG, where the limited material allowed to sample only 1 replicate) and was directly frozen in liquid nitrogen to preserve the DNA.

The ACLs were not directly frozen into −80°C which may have influenced and reduced the immunohistological stainings of collagen type 3. Furthermore, the evaluation of the H&E and Lendrum's Masson's trichrome was qualitative, though standard histology is commonly used as it provides a representative impression.

The blood culture was directly frozen at the hospital without further subculture.

Salivary specimens were directly frozen without processing or culture at −70 degrees Celsius.

Crystals grew as thin plates to full size (≈0.2 mm ×0.1 mm ×0.02 mm) in 3 weeks and were directly frozen out of mother liquor into liquid nitrogen.

Suitable crystals were washed in 100 mM MES pH 7.0, 30% (v/v) ethylene glycol, 1.15 M Na-citrate and directly frozen in liquid nitrogen.

Crystals grew as tetragonal rods to full size (≈0.5 mm ×0.1 mm ×0.1 mm) in 10 days and were directly frozen out of mother liquor into liquid nitrogen.

Cells were sorted by flow cytometry using an automatic cell deposit unit (ACDU) in dry 96-well plates and directly frozen at -80°C overnight.