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To construct a phaZ3 deletion allele, two sets of primers were used to amplify two ~500 bp regions of DNA, directly flanking the phaZ3 open reading frame.
Therefore and because of this bias, we studied only one CpG site directly flanking the pyrosequencing primer.
Interestingly, a manual alignment of VirB7Ti with the putative Rickettsia VirB7 and the ComB7 proteins of H. pylori and C. jejuni revealed a conserved motif "[KI]KSP" directly flanking the second conserved Cys in the latter three taxa (Figure 8B).
A similar analysis was carried between D10S1223 and D10S1248, the two markers directly flanking the linkage peak in 10q26.3, between positions 129,150,822 and 130,982,363 in chromosome 10.
We applied this method to the 6q26 region between D6S476 and D6S305, the two markers directly flanking the linkage peak seen in the region, which corresponds to the 10.48 MB band between positions 151,685,574 and 162,165,587 of chromosome 6.
The target site duplication was examined in the genomic sequence directly flanking the LTRs.
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It is made up of tandem repeats of a 234 bp A-T rich motif, called the major satellite, constituting a domain which directly flanks the inner centromere.
Furthermore, ENU mutations were often directly flanked by G or C nucleotides.
These observations suggest that HDR is more robust when the homology arms directly flank the cut site.
For candidate L1 loci directly flanked by substantial stretches (more than 1 kb) of repetitive genomic sequence, primers were designed manually.
Support for this hypothesis stems from the observation that IRs directly flank SIX12 and the SIX3/SIX5 mini-cluster, although the transposase recognizing these IRs remains unknown.
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