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Determination of RNA concentration and purity were performed on a Nanodrop 1000 (Thermo Scientific, Wilmington, DE) directly before using the purified RNA for RT-qPCR.
Similar(59)
The polymerization mixture was produced by mixing the solution A and B in the proportion of 9 parts A and 1 part of solution B directly before use.
Always shake before using.
Test always before using.
Use a soft, dry cloth and pour some of the cleaner directly onto it before using the cloth to apply the cleaner to the glass.
The plate was washed 5 times with PBS complemented with 0.5 mM MgCl2 and 1 mM CaCl2 directly before use.
Bromophenol blue solution was prepared directly before use.
Oxaloacetate was dissolved directly before use to avoid decarboxylation.
Frozen microtubules were thawed at 37°C directly before use.
THF was distilled over Na/benzophenone directly before use.
THF used for anhydrous reactions was distilled from potassium/benzophenone directly before use.
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