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Notably, RT-PCR data is suggestive for the microarray to underestimate changes in the gene expression even though both methods supported the general directions of changes.
In contract, the two-sided MADe method intends to select sample-level differentiated genes from both directions of changes (e.g., pooled highly expressed genes and lowly expressed genes together) in each sample.
As shown by these formulae, the directions of changes in sensitivity and in specificity are reversed, and the directions of changes in PPV and in NPV are reversed.
Even so, we did find significant differences between formats, in particular we identified clear directions of changes for two formats.
We then investigated whether the directions of changes (i.e., hyper- or hypomethylation) are the same when comparing Turner or Klinefelter samples to either male or female samples.
Although the directions of changes in the 50 vs 180 ATU hybridizations were largely similar in both healthy and M74 specimens, there were differences in the magnitude of up/downregulation of many genes.
Similar(51)
The directions of change were similar in both blood and urine LH.
The arrow indicates the direction of changes with the spectra.
Depending on the direction of changes, two separate analyses are performed.
They've been able to pick the direction of changes in the curve only 28% of the time.
The direction of changes did not correlate with tumor grade of differentiation (Fig. 1A).
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