A second round of amplification is then performed via primer extension [ 101] to generate double-stranded cDNA that can then be digested and directionally cloned into an appropriate vector.
cDNA encoding latherin, excluding the leader peptide, was amplified by PCR using appropriate oligonucleotide primers (Table S2), and directionally inserted into NcoI, XhoI sites of the pET32a expression vector, and the resulting 'LathpET32' plasmid was transformed into BL21 DE3 pLysS cells.
Additionally, the vignettes were modified iteratively until they were both reliable in eliciting appropriate responses from a beta panel of diagnosticians, as well as directionally accurate (i.e. until each vignette elicited consistent diagnoses that matched that of the psychometrically evaluated actual cases they were derived from).
Information from these tests contributes to making a directionally specific diagnosis, which should make it possible to design a retraining program and provide more specific advice on appropriate physical activity, including measurement of the effect on LMC.
DOJ v Wall Street: from zero to hero This rampage is great … directionally.
"Not how I would have put it," he explained, "but directionally right".
"It does not appear to be a home run, but it is directionally positive".
Dirk Meyer, A.M.D.'s president and chief executive, called the performance "directionally correct," but not entirely satisfactory.
It is just the type of information that a directionally challenged man needs.
"The work we're doing is really, directionally, the right thing to do," he said.
