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The forward template (S1, 109 nt) contained an Eco RI restriction site in a 5'-3' direction (underlined in the S1 sequence) and a T7 promoter sequence downstream (shown in bold in the S1 sequence), separated by four spacer nucleotides.
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Twhirl and FriendFeed provide a reasonable way to monitor the Twitter side by side with the fast Identi.ca stream, but the reverse direction underlines the handcuffs Twitter has imposed.
The design was exquisite, Susannah Fielding and Pippa Bennett-Warner were a glorious double act as the quarry who are really in control and Jonathan Goddard's movement direction continually underlined the play's joyous verve.
Language cannot simply be there to give us directions or underline the action.
The preferential involvement of anterior STS in view-invariant representations of gaze direction was further underlined by the analysis of left STS, which identified the anterior region alone.
The direction, by Mark Steven Johnson, underlines every nature-bound, macho-duel element of Evan Daugherty's script.
"Big Bill," which opened last night at the Mitzi E. Newhouse Theater under Mark Lamos's oddly flaccid direction, scrambles chronology to underline this fall from grace.
You would think that Formula 1 was an organisation that, self-evidently, did not need to underline the direction in which it was moving.
Just to underline the direction in which the ladies game has long been heading, another South Korean, Ryu So-yeon, and one of Korean extraction, the New Zealander Lydia Ko, tied for third.
The literature sources on dilatometric studies of aluminium with nanotubes describe TEC measurements taken only in one unique direction, whereas our studies underline the importance of the directions used for measuring.
In this review, an overview of recent findings that highlight the material properties that are important in guiding MSC fate decisions is presented, with a focus on underlining gaps in our existing knowledge and proposing potential directions for future research.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com