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The difference between maps and other data-conveying graphics is that maps exist to represent spatial relationships — direction, sequence and distance.
With the proposed design technique, Earth Moon transfer trajectory is successfully designed with the plasma thruster having a thrust direction sequence of "fixed-varied-fixed" and a thrust acceleration sequence of "constant-variable-constant".
If and only if the single strand had a high-quality read, a single direction sequence was used.
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Purified PCR products were double direction sequenced using an automated DNA sequencer (ABI 3730, Applied Biosystems).
To verify the quality of the library, a portion of the final DNA products were cloned into a plasmid vector and randomly selected 192 clones for one direction sequencing on the ABI 3730 sequencer (Applied BioSystems, Foster City, CA).
Here we describe a strategy that provides full bi-directional sequencing coverage using single forward and reverse direction sequencing mixes.
For unigenes with sequence direction, sequences from the 5' end to the 3' end were provided.
Forward and reverse direction sequences were reduced to variants by collapsing sequences that were 100% identical to each other, using the CD-HIT webserver [ 34].
The restricted plasmids or PCR products were bidirectionally sequenced on an ABI 3730XL DNA sequencer (Applied Biosystems, Foster City, CA, USA) using the BigDye terminator cycle sequencing ready reaction kit (Applied Biosystems); as a result, one direction sequencing entirely covered another direction except for samples of 2010.
Stacked reads i.e. redundant reads sharing the same start and end coordinate, sequencing direction, and sequence were merged into one.
PCR product was visualized on agarose electrophoresis and submitted for both directions sequencing at the Research Technology Support Facility (RTSF), MSU.
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