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Research efforts in recent years have been directed toward actively controlling the direction of translocation of microtubules on a kinesin-coated glass surface with E-fields (electric fields), opening up the possibility of engineering controllable nanodevices that integrate microtubules and motor proteins into their function.
Lastly, we studied the effect of cell line, Ca2+ concentration, chemical inhibitors, and cell morphology on translocation and found these conditions affected the rate, extent and direction of translocation.
We report that the underlying DNA sequence can control the initial direction of translocation, translocation distance, as well as the new positions adopted by nucleosomes upon enzymatic mobilization.
For example, nucleosomes positioned by DNA sequence can control the initial direction of translocation, translocation distance, as well as the new positions adopted by nucleosomes in response to chromatin remodeling machines [70].
2) The reviewers are concerned that the model presented by the authors may have the direction of translocation backwards.
We show that SpoIIIE makes multiple essential contacts with phosphates on the 5'→3' strand in the direction of translocation.
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For this, we focused on the role of management-related translocation of bears, for which we were interested in quantifying the direction of translocations and difference in habitat availability among management units.
The voltage applies a force along the direction of the translocation, which impedes the rate of the forward translocation (r1) and increases the rate of the reverse translocation (r2).
Furthermore, its quasi-central position did not allow us to determine the direction of nucleosome translocation.
Nevertheless, we found no evidence that DNA end binding of RSC influences the initial direction of nucleosome translocation.
It was proposed that the physical interaction between BLM and Rad51 serves to recruit BLM to sites of recombinational repair and possibly load BLM onto Holliday Junctions in a particular orientation that would then dictate the direction of junction translocation.
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