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From these observations it is also concluded that the direction of spindle migration was consistently toward the spindle pole containing the greater cluster of MTOCs, judged by Cep192 intensity.
These measurements also suggest that the direction of spindle movement can be predicted before migration occurs, and correlates with the direction in which the homologue pairs experiencing greater C Kt stretch are facing.
Because F-actin is as well required for the anchoring of mitotic spindle to the cell cortex and moreover to establish the direction of spindle movement (Woolner and Bement 2009), it is plausible that the excessive amount of overexpressed EN-actin (which may form filaments during mitosis) prevents correct spindle positioning and manifests in chromosome segregation errors.
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As spindle orientation can be reliably inferred from chromosome shape in wild-type oocytes, we initially attempted to use FISH labeling of the exchange chromosomes 2 and 3 to indicate the directions of the spindle poles.
Frequently, the shortened polar axis coincides with the direction of the mitotic spindle extending between anaphase clusters (Video 1).
Make sure the direction of the spinning hand spindle (drop spindle) always stays the same.
Also, it is found that higher travelling velocity of the tire leads to higher resonant amplitude of spindle forces in the vertical direction.
The patterns have been generated on surface by particular positioning of workpiece and tool, milling passes in different directions, and as well as special angular position of spindle in typical vertical milling machine.
Immediately prior to collapse of the spindle (Fig. 1B, 0 min, arrows show direction of impending collapse), mitochondria were seen flanking the spindle midzone region.
Spindle poles attach to mature centrosomes; they control the position of the central spindle and, hence, the direction of the last stage of the cytokinesis cleavage furrow (Nicholas et al., 2010).
Lists, illustrations and the significance of spindle count on Windsor chairs.
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