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2) The data on the direction of spike propagation in VSI where presented appear as digressions.
Major revisions: The material on the direction of spike propagation in VSI seem unnecessary digressions in a data packed paper.
The difference in the synaptic action in the pedal ganglia may affect the direction of spike propagation between them.
The direction of spike propagation in VSI axon was predictive of susceptibility of the swim motor pattern to PdN6 disconnection.
We thank the reviewers for pointing out about the temporal order of the direction of spike propagation.
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In Animal 2, VSI was already exhibiting orthodromic action potentials even before blocking PdN6, showing individual variability in spike initiation zones (see 'Direction of VSI spike propagation predicted the extent of motor impairment').
(C ) Overlaid spike-triggered impulses for each burst recorded from PdN6 in three individuals show variability in the direction of VSI spike propagation (C i, antidromic; C ii, mixed; C iii, orthodromic).
(D ) The direction of VSI spike propagation in PdN6 was predictive of the extent of impairment after PdN disconnection.
As a next step towards the introduction of EHG analysis as a clinical tool, we propose an automated method for EHG conduction velocity estimation for both the speed and direction of single spike propagation.
Source data for panel D. DOI: http://dx.doi.org/10.7554/eLife.02598.020 10.7554/eLiFigure98 sourcegure 8—source data 2. Source data for figure supplement 1 panels B and C. DOI: http://dx.doi.org/10.7554/eLife.02598.021 10.7554/eLiFigure98 figuregure 8—figure supplement 1. Inter- and intra-individual variation in the direction of VSI spike propagation during the swim motor pattern.
The extent of impairment by PdN6 disconnection, shown as the percent change in the number of VSI bursts per swim episode, is plotted in three groups categorized by the direction of VSI spike propagations (black, all VSI bursts were antidromic; gray, mixed; white, all bursts were orthodromic).
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