Sentence examples for direction of sequence from inspiring English sources

In particular, since the direction of sequence synthesis is 5′→3′, all sequence reads with tags inversely directed (3′→5′) were considered chimeric artefacts produced during the multidisplacement amplification activity of the Phi29 DNA polymerase [ 26, 27] and can be quantified.

Identification of centromere will provide valuable information regarding the location of inversion and direction of sequence expansion at the early stage of papaya sex chromosome evolution.

If so, the direction of sequence changes in H1N1/09 in the near future is predictable, and this should be testable because of the high mutation rate and short generation time of influenza A viruses.

At this stage we also removed variants that were only detected on one direction of sequence read (either forward or reverse), as these are likely to represent sequencing artifacts.

Rodríguez-Moreno et al. [ 60] suggested that the extent of similarity indicates the massive induction of nuclear sequences into mitochondrial DNA; however, no attempts were made to determine the direction of sequence transfer.

Crick was discussing solely the direction of sequence information flow between the two classes of macromolecules and his specific conclusion is still valid: nucleic acid sequence information can be read into proteins or copied into each other (DNA → RNA or RNA → DNA) but protein sequences cannot be reverse-read into nucleic acid sequences.

PCR primers in the opposite direction of sequencing primers were biotin labelled.

(2013) point out that even the choice of MID code used to separate samples, direction of sequencing and quality filtering have distinct, unpredictable and inconsistent impacts on the recovery of sequences and these interact with each other.

ESTSCAN software [ 48] was also used to determine the direction of sequences that did not align to any of the databases, and CDSs shorter than 150 bp were removed.

B: Fig. 2-5 & 7: please keep coherent direction of sequences e.g. GC/GC (5'-3'/5'-3') in Fig. 2 vs Fig. 4 GC/CG, or coherent naming of modification: Fig. 2 – no indication which nucleotide is modified, Fig. 4: C', Fig. 5: (5-mC), if mis-understood – please clarify.

Since 454 sequencing is not directional, we used the 5' adapter sequence to determine the direction of each sequence read.