For GST, the fold-change was smaller, but the direction of regulation (4.71-fold up-regulated NS) was similar to that determined by microarray (15.24-fold 15.24-folded NS).
A fortiori, analyzing the direction of regulation (induction or repression) of each regulated molecule seems pointless in such large eukaryotic systems, and in the state of our current knowledge.
Diurnal variation was observed for all features of transcriptional regulation, i.e., number of regulated transcripts, fold-change value, and direction of regulation.
Diurnal variation was demonstrated for several features of transcriptional regulation, i.e., total number of significantly regulated transcripts, fold-change and direction of regulation, and transcript-associated cellular function.
Phytozome locus name, gene names and description, co-regulated module membership, timestamp and direction of regulation are indicated.
However, a large number of the probe sets that are differentially regulated in WT mice showed the same direction of regulation by prednisolone in GRdim mice, but did not meet the cutoff value of 0.01 for the p-value.
However, the direction of regulation for plastic responses was highly uniform for genes regulated by copper-binding transcription factors in the relevant environment (fig. 5) and more variable for genes involved in metabolism (fig. 7).
By real-time RT-PCR (n = 4), two of the four genes, Stard7 and TepII, were significantly differentially regulated (p = 0.029 and 0.014, respectively); the direction of regulation was the same as determined by microarray.
The same analysis was carried out separately for all the differentially regulated gene sets and by taking into account the direction of regulation (repressed or activated).
From the 20 sRNA genes that showed media-dependent regulation, seven were regulated in both THY and BHI, and showed the same direction of regulation compared to CDM.
