For GST, the fold-change was smaller, but the direction of regulation (4.71-fold up-regulated NS) was similar to that determined by microarray (15.24-fold 15.24-folded NS).
Diurnal variation was observed for all features of transcriptional regulation, i.e., number of regulated transcripts, fold-change value, and direction of regulation.
Phytozome locus name, gene names and description, co-regulated module membership, timestamp and direction of regulation are indicated.
By real-time RT-PCR (n = 4), two of the four genes, Stard7 and TepII, were significantly differentially regulated (p = 0.029 and 0.014, respectively); the direction of regulation was the same as determined by microarray.
The same analysis was carried out separately for all the differentially regulated gene sets and by taking into account the direction of regulation (repressed or activated).
From the 20 sRNA genes that showed media-dependent regulation, seven were regulated in both THY and BHI, and showed the same direction of regulation compared to CDM.
Gene expression is either induced or repressed while direction of regulation of the biological processes is not accessible.
Among these 34 transcripts, 25 were upregulated and all transcripts showed no change in direction of regulation with changing exposure condition.
The invited participants were selected because of their broad experience as regulators, practitioners, or academics in international securities transactions, assuring a wide spectrum of views on the future direction of regulation.
In the kidney medulla, Cldn11, Psca, and Slpi were consistently down-regulated, and Klk1b5 was consistently up-regulated, while Hmgcs2 and Ubd were the only genes that showed changes in the direction of regulation (Fig. 5b).
Overall, significant detection of potential biomarkers and signature genes was validated at each time of day, although direction of regulation and fold-change differed between morning, noon, and afternoon.
