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However, using only SNPs with an MAF lower than 0.15 in the sequenced population, and under the assumption that the direction of mutation is from the reference allele to the minor allele, the mutational spectrum of CRt was investigated.
So we could unambiguously find the direction of mutation".
Finally, we examined whether the direction of mutation (gain vs. loss) was related to repeat number.
The influence of STR motif on the direction of mutation still has to be investigated.
The shape of the clone alignment is more like a "bush" than a "tree", showing no clear single direction of mutation.
These 12 were removed from the data set, resulting in 167 SNPs in which the direction of mutation could be confidently determined (fig. 1 and table 1; table S1, Supplementary Materials online).
Similar(52)
They are introduced to mutation as the generator of the variation present in the population, and they observe that the direction of mutations is random (offspring may have shells that are either thicker or thinner than their parents), and that the presence of crabs does not affect the direction of mutations.
Ancestral alleles were obtained from dbSNP (build 128) to check direction of mutations for SNPs causing changes between amino acids and stop codons.
The direction of mutations within BGa showed strong deviation toward the W→S type.
The identities of the ancestral bases and the direction of mutations were inferred from human-chimpanzee genomic alignment.
We used the genome of the rhesus macaque (Gibbs et al. 2007) as an outgroup to the human and chimpanzee genomes to polarize the direction of mutations.
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