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To determine whether Tbx6 and Wnt3a interact genetically, we intercrossed Tbx6tm2Pa and Wnt3a heterozygotes and examined the direction of heart looping and somite morphology of double heterozygous offspring.
Homozygous mutants, but not compound null/hypomorphic heterozygotes, show randomization of the direction of heart looping and independent alterations in the direction of embryo turning.
At E10.5, all Tbx6; Wnt3a double heterozygotes (n = 18) and all Tbx6 single heterozygotes (n = 19) displayed normal heart looping and somite formation, indicating that there is no genetic interaction between Wnt3a and Tbx6 with respect to direction of heart looping or somite segmentation.
Disruption of Nodal signalling within the heart field by the knock-down of southpaw (spaw) /nodal, or randomization of spaw/nodal expression due to immotile cilia disrupting nodal flow in switch hitter mutants resulted in a randomization in the direction of heart morphogenesis.
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But it's highly unlikely that it'll go in the direction of "Electra Heart".
Overall, I am a firm believer in making small sustainable moves in the direction of my heart's desire knowing that all will work out in the big picture.
You should always move in the direction of the heart.
All of your brush strokes should be long sweeping strokes in the direction of your heart.
Lean your wedge to target and club end point to the direction of your heart.
During or after the hot water soak, perform effleurage with oil on the lower leg and foot (avoiding the sting site itself) in the direction of the heart.
The musical score for Senna is so powerful, it evokes an exquisite chest-pain, somewhere in the direction of our hearts.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com