Sentence examples for direction and sequence from inspiring English sources
The direction and sequence authenticity of the above constructs were validated by restriction analysis and direct sequencing.
Stacked reads i.e. redundant reads sharing the same start and end coordinate, sequencing direction, and sequence were merged into one.
We employed the ancestral area method from Bremer [ 39] to infer the direction and sequence of island and mainland colonization of C. flaveola in the West Indies.
Our results suggest that use of WGS can reduce ambiguity about potential transmission events in hospitals and consequently inform infection prevention efforts about the direction and sequence of transmission.
Adjacent channels are spaced approximately five centimeters apart in the cross-track direction, and sequences (or scans) are taken at approximately 1-cm down-track intervals.
Adjacent channels are spaced approximately 5 cm apart in the cross-track direction, and sequences (or scans) are taken at approximately 1 centimeter down-track intervals.
The PCR products were cloned in pCRII-TOPO vector (Invitrogen), digested with restriction enzymes to identify the correct direction and sequenced (Base Clear lab services) to verify that the final products correspond to the genes of interest by aligning them to genes previously described using the VectorNTI program.
The seven loci were sequenced in both directions, and sequence data were checked manually for positions of homozygotic or heterozygotic polymorphisms.
Sub-clones were sequenced with the universal priming sites on the vector in both the forward and reverse directions and sequence data collected on an ABI 3730xl DNA Analyzer.
ESTScan (Iseli et al. 1999) was used to scan unigenes that could not be aligned to any database, producing nucleotide sequence (5′ 3′) direction and amino sequence of the predicted coding region.
Transcript Unigenes unaligned to any of the protein databases were analyzed by a software named ESTScan [ 82] to determine the nucleotide sequence (5'-3') direction and amino sequence of the predicted coding region.
