Exact(25)
These studies indicate that SATB1 could mediate the function of MAR elements in directing the expression of β-globin genes.
Sequences directing the expression of Diphtheria toxin A in mouse ES cells were cloned into pBluescript (pgk-neo) [38] to give the plasmid pBSneoDTA.
We ligated the PCR products into SalI/NotI-cut pACT, a plasmid directing the expression of proteins fused to the VP16 activation domain (Promega).
Adenoviral vectors directing the expression of IκB (AdIκBα), a mutated IκB as a NF-κB inhibitor, and GFP (AdGFP) as viral vector controls were used [9], [10].
Therefore, the crn-6 promoter is capable of directing the expression of NUC-1 to mediate degradation of bacterial DNA in intestine but the level of NUC-1 expression appears to be critical for this function.
To confirm that the effects observed in the 11635 homozygous mutants are due to a perturbation of the hmgcr gene, we genetically rescued the phenotype by directing the expression of the the hmgcr gene in the 11635 homozygous mutant.
Similar(35)
Most notably when co-expressed in planta with ATAF2 this 30-bp sequence directed the expression of a GUS reporter construct.
GUS assays proved that the isolated promoter was able to direct the expression of the reporter gene in transgenic tobacco.
The CrPV IGR contains an internal ribosomal entry site (IRES) that directs the expression of structural proteins.
Using the commercial Drosophila expression system (DES), we transfected S2 cells with plasmids designed to direct the expression of secreted recombinant HER2 ECD and DIV proteins.
Such element was designed to direct the expression of a reporting gene (firefly luciferase) through the activation of Drosophila melanogaster hsp22 promoter.
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