The author, Errol John, was so appalled by the shoddy treatment of the play that he vowed he'd never allow it to be staged again in British theatre until he was trusted to direct it himself – 27 years later he directed it successfully at the Theatre Royal Stratford East.
In summary, this study demonstrated that directed evolution successfully yielded a CL Pgp that is well expressed, completely active in in vivo yeast assays, and can be readily purified.
These results demonstrate that random recombination of NBD1/linker and NBD2 Cys mutations, identified by directed evolution successfully yielded several Pgp proteins that maintain high levels of activity with six native Cys residues removed.
These results show that directed evolution successfully produced a novel CL Pgp that bypassed the limitations of the previously constructed mouse and human CL Pgps [ 16, 17, 22, 30], as it is both active in yeast functional assays and can be readily purified in milligram quantities.
The more often you change them, the less people can direct link successfully from you.
In addition, successfully directed modification and special evolutionary status of FSA imply its directional reconstruction potentials for bioethanol production, as well as for other industrial applications.
Bass successfully directed and produced short animated films, television openings and commercials, live documentaries, and features.
By 1858, having overcome extended opposition, Philaret successfully directed the translation of the Bible into modern Russian.
Having successfully directed period pieces like "The Light in the Piazza" and "South Pacific," Bartlett Sher will soon be directing, not surprisingly, another period piece.
Even in his mid-30s, with a thicker waist and a few more rings within those aged tree-trunk thighs he successfully directed traffic in Liverpool's midfield.
