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It is shown that additional quantitatively directed site exploration during the design phase could have prevented the remedial failure that was caused by missing a geologic body having high hydraulic conductivity at the south end of the barrier.
Ser109 corresponds to a basophilic site (K/R-X-X-S/T), while Ser288 fits an acidophilic motif (S/T-X-X-D/E), and Ser310 corresponds to a proline directed site (S/T-P).
Since DYRK1A is a proline- directed serine/threonine kinase and serine 262 is a non-proline directed site on tau, these effects of DYRK1A may result from indirect effects through additional kinases (see Discussion).
To examine the presence of bar [ 11, 12] or the LTB gene [ 13] at a directed site in the chloroplast DNA after homologous recombination in transplastomic tobacco plants, putative transformants were screened by PCR analysis.
Thirty six Web sites (60%) were linked to state registries for consent to organ donation; the other 24 Web sites directed site visitors to fill out state donor cards or include signed consent on the driver licenses' issued by the Department of Motor Vehicle (DMV).
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Alternatively, NCL with cysteamine (11) provided access to C-terminal aminoethanethiols, which have proven useful in recent applications of disulfide directed, site-specific ubiquitination of proteins.
A point mutation, leading to substitution of the Cys codon at positions 56 or 178 to Ser codon was introduced into the alr4641 ORF by PCR directed site-specific mutagenesis using overlapping PCR as described earlier [ 25].
Mrs. MacInnes, 33, works in the marketing department of 6FigureJobs.com, an online job board in Darien, Conn., where she directs site content.
The membrane directs site and substrate specificity by rhomboid proteases.
This matrix serves to organize cells in space, to provide them with environmental signals to direct site-specific cellular regulation and to separate one tissue space from another.
A 9bp direct target site duplication was found at the insertion site.
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