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It is easy to see that the MCRS model might be very sensitive to this detail: template directed replication is a dynamical issue requiring closer study.
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Here, we review the different viruses able to direct replication and gene expression in yeast and discuss their main contributions in the understanding of virus biology.
Members of the Parvoviridae family all encode a non-structural protein 1 (NS1) that directs replication of single-stranded viral DNA, packages viral DNA into capsid, and serves as a potent transcriptional activator.
Although about 350 ARS have been estimated in the genome of S. cerevisiae, only a subset of them appears to direct replication initiation in the S-phase [26], [41].
For years, it was extremely difficult to publish a direct replication, or a failure to replicate an experiment, in a good journal.
After calibrating the gel system with this well-studied rolling circle replication reaction, we then analyzed the in vivo replication directed by a T4 replication origin cloned within a plasmid.
Given the unexpected direction of the interaction of smoking with BMI, we attempted a direct replication of this finding using data from the independent ESTHER cohort, in which we had first described the smoking × alcohol interaction [13].
In the first stage, we attempted to replicate 20 of the 29 exact published SNPs (i.e. direct replication) available in the HUFS dataset.
However, the film is reportedly not a direct replication of events.
We conducted a direct replication of this imaging study in a separate behavioral experiment (n = 102).
Evidence reviews confirm that while lessons should be learned from successful initiatives, direct replication is seldom entirely effective.
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