Sentence examples for directed point from inspiring English sources

The vector encoding wild-type Myc was used as a template for oligonucleotide directed point mutagenesis utilizing a QuickChange II kit according to the directions of the supplier (Stratagene, Inc. LaJolla CA).

In addition to previously established high efficient knock out and knock in strategies in Toxoplasma, the site directed point mutation technique presented in this manuscript provides another powerful tool set for T. gondii research.

It's directed point blank at you.

Since the cas1 gene contains an internal NcoI restriction site, a site directed point mutation was introduced by overlap extension PCR with primer pairs cynT1-GFP-f/T1-Mut-r and T1-Mut-f/cynT1-r (Table S1).

In this paper, we study the practical issues on the design of a new communication subsystem, named Directed Point (DP), on a server cluster with Gigabit Ethernet connection, with the goals of achieving high performance and good programmability.

Directed point mutations of "portal entrance" residues, A47 and D48, reversibly regulate sphingosine access to the hydrophobic pocket via a mechanism that could involve homodimerization.

Others have directed pointed insults akin to what might be heard in a rap song.

Time and again, Mr. Gore directed pointed and detailed questions at Mr. Bush, who rarely talks about Medicare.

All the links are directed, pointing from the higher-ranked to the lower-ranked occupations.

We describe two PCR-based methods for directing point mutations into the yeast genome such that the final product contains no other disruptions.

Gregory Doran, who was directing, pointed out that with these jump cuts between scenes it's almost like a movie.