The RDF data model is a directed labeled graph, in which nodes are Resources or Literals, and edges are Properties.
RDF is a model of directed, labeled graphs that use a set of triples.
Although less expensive, we and other colleagues have found that indirect labelling techniques are not always reliable compared to direct labelling methods.
Per microarray, 20 μg mRNA, pooled from 2 livers, was reverse transcribed with Cy3-labelled dCTP (Perkin Elmer, Boston, USA), using the Agilent Fluorescent Direct Label Kit.
Total RNA (20 μg) was labeled using a Fluorescent Direct Label Kit (Agilent Technologies) and simultaneously reverse transcribed into cDNA.
The probe bears a direct label (e.g. fluorescein) or a tag for subsequent detection (e.g. biotin).
Since the latter half of the last decade, Trago's divided his time between Rush Hour, another Amsterdam institution, and his own Voyage Direct label.
The Agilent fluorescent direct label kit was used to generate labeled cDNA for steady state experiments from 10 µg of total input RNA.
In Agilent experiments, 10 μg of total RNA was used for labeling by Agilent Fluorescent Direct Label Kit (Agilent Technologies, Pato Alto, CA, USA).
Twenty microgram total RNA was reversely transcribed and fluorescently labelled using the Agilent Fluorescent Direct Label Kit (G2557A), according to the manufacturer's instructions.
Or, better yet, look for a "Farm Friendly Direct" label.
