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The directed introduction of point mutations into ARTD10-encoding plasmids was achieved by using the QuickChange Site-directed mutagenesis kit (Stratagene, La Jolla, CA, USA) according to the manufacturer's instructions.
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Remember that women respond better to direct introductions, so keep this in mind.
Here to provide clarity and direction is Ms. Lena Dunham, who wrote and directed this introduction to the iPhone edition, and brought along two partners in crime, Jon Hamm and Alex Karpovsky: Download this week's issue for free.
The answers came in a visit to the Chrysler Technology Center in Auburn Hills, Mich .Fabio DiMuro directed the introduction of the European 500 and took the lead in the United States for the rollout of the Nafta version.
Direct introduction also enables them to escape the toxic effects of chemical protectants.
Aerosols pneumatically generated have characteristics that prevent their direct introduction into the plasma.
Recent progress in direct introduction of fluorinated groups on alkenes and alkynes by means of C-H bond functionalization.
Cases often arise where dehumidifying requirements result in an air temperature too low for direct introduction into the room.
Al Cu pillared clays were prepared by direct introduction of Al Cu pillaring solution into the dilute bentonite suspension.
However, direct introduction of aqueous solution into the microplasma for the detection of metals remains problematic due to plasma instability.
"This is a direct introduction for voters to the governor," said Mr. Legittino, running through last-minute changes a few hours before the Lancaster rally.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com