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We found that these cells were able to differentiate into astrocytes upon directed differentiation.
Most established methods of directed differentiation result in heterogeneous populations of differentiated cells.
These are pluripotent stem cells (PSCs) (through directed differentiation) and pancreatic non-endocrine cells (through directed differentiation or reprogramming).
Spence, J.R. et al. Directed differentiation of human pluripotent stem cells into intestinal tissue in vitro.
Liu, Y. et al. Directed differentiation of forebrain GABA interneurons from human pluripotent stem cells.
Douvaras, P. et al. Directed Differentiation of Human Pluripotent Stem Cells to Microglia.
Kaufman, D.S. et al. Directed differentiation of human embryonic stem cells into hematopoietic colony forming cells.
Yan, Y. et al. Directed differentiation of dopaminergic neuronal subtypes from human embryonic stem cells.
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Clonal lineage analysis of the differentiating progenitors is needed to clarify the exact mechanisms governing the directed-differentiation.
Our finding also opens the door for the development of reprogramming and directed-differentiation methods based on long-RNA transfection.
Swift, J. et al. Nuclear lamin-A scales with tissue stiffness and enhances matrix-directed differentiation.
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