The first directed component is the direction that connects hMSC to hOST.
Most diagrams illustrate the presence in all the samples of a northward directed component with maximum unblocking temperatures of 250°C which may represent from 50% to 95% of the NRM.
Figure 2 (right) plots the third component versus the directed component.
Thus, the first directed component provides a characterization of how "osteoblast-like" the samples are.
The directed component can then be approximated as proportional to the difference in the receptors bound between the left and right ends of the tip.
The remaining components are selected to maximize the explanation of the remaining variability subject to the constraint that they be uncorrelated (orthogonal) with each other and with the directed component.
The directed component specifies the mean angle that a theoretical pollen tube would turn in response to a gradient of the attractant, and the random component adds a random angle chosen from a Gaussian distribution to this mean direction.
The directed component accounts for 26% of the variability while the second, third, and fourth components account for 41%, 22%, and 10% of the variability respectively in the first five experiments.
The directed component accounts for 26% of the variability while the second, third, and fourth components account for 41%, 22%, and 10% of the variability, respectively, in the first five samples.
The coefficients for each experiment may be plotted against each other, as in Figure 2. By construction of the directed component, the further right a population falls the closer it is to hOST.
The trends in Fig. 3B do not indicate any saturation; furthermore, initial fits of our data to this case further suggested that the directed component was well modeled by receptors far from saturation, where the ligand binding is stoichiometric.
