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An automated liquid handling unit directed buffer, sample, amplification and regeneration solutions to the sensor and metered the flow rate.
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Net worth serves as a direct buffer against decreases in the value of the bank's assets, protecting the depositors.
In this light, Hsp90, the prototypical phenotypic capacitor, may not be representative: typical phenotypic capacitors are not direct "buffers" of variation, but are simply genes encoding central cellular functions.
This information is used to allocate send and receive buffers and to direct each entry in a tasks send buffer to the correct MPI task in a round of all-to-all communication.
The membranes were incubated for 1 h in TBST buffer containing antibodies directed against the β1, α1 (Sigma) and α2 [34] subunit of soluble guanylyl cyclase.
Cultures were treated as indicated above and collected using Passive Lysis Buffer (Thermo Fisher Scientific) as directed by the manufacturer.
Crosslinking was then reversed in direct elution buffer (10 mm Tris-HCl, pH 8, 0.5% SDS, 5 mm EDTA, pH 8, 300 mm NaCl) at 65 °C O/N.
The nuclear lysates were prepared in direct lysis buffer as previously described [ 5].
Hybridizations were carried out overnight at 50 °C in AlkPhos Direct hybridization buffer.
The aqueous phase was transferred into a new tube and added to 30 µl of Direct Purification Buffer and 25 µl of Wizard PCR Preps DNA purification resin (Promega) per oocyte.
The immunoreactive chromatin was recovered in 400 μL of ChIP direct elution buffer (10 mM Tris HCl, pH 8.0, 300 mM NaCl, 5 mM EDTA and 0.5 % sodium dodecyl sulfate).
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