Sentence examples for directed as described from inspiring English sources

Secondly, the antibodies were preabsorbed with the corresponding peptide against which they are directed as described previously [ 24]; under this condition staining was completely blocked.

To ensure specificity, the following control experiments were performed: firstly, the primary antibody was omitted; and secondly, for COX-2, the antibodies were preabsorbed with the corresponding peptide against which they are directed, as described previously [ 24].

Cell proliferation was assessed by direct counting as described by Liu et al. [ 19].

The resulting integration vector was inserted into the bacterial attachment (attB) site of E. coli using a helper plasmid, pAH121, expressing the phage integrase, by direct transformation, as described by Chiang et al. [ 26].

The internal BamHI restriction site in the PTP sequence was removed by site directed mutagenesis as described above (see Table S2 for primers).

Therefore, K189 and D159 of the Walker A (GxxxxGKT) and B (DExx) motifs respectively in rNSs were mutated to alanine by site directed mutagenesis as described in the methods section.

The CHEK2 H371Y mutation was detected by polymerase chain reaction (PCR) followed by denaturing high-performance liquid chromatography (DHPLC) and sequencing or directed sequencing as described previously [ 21].

Dried filter papers were kept at −20°C until the direct agglutination test was performed as described elsewhere.

The D174H mutation was introduced by PCR site-directed mutagenesis as described previously [55].

The plasmid containing WT NPM-ALK was used as a template for in vitro site-directed mutagenesis, as described above.

PDIA6 (135-421) C171S, PDIA6 (135-421) C174S, and PDIA6 (135-421) C174A mutations were made using 'Round the Horn' site-directed mutagenesis as described previously (Chutkow & Lee, 2011).