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Based on observations of a dose-dependent yeast response to furfural, we developed a directed adaptation method and generated an adapted strain Y7-1.
A directed adaptation method was developed and applied.
To our knowledge, this is the first report of the directed adaptation of a filamentous fungus for a complex phenotype via continuous culture.
The availability of a robust selection method for filamentous fungi is an important new tool for directed adaptation that could have significant applications ranging from improved industrial strains to the examinations of the mechanisms that underlie evolution in eukaryotic organisms.
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