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Tandem mass spectrometry analysis was done with a MicroQtof (Waters) using data directed acquisition.
Samples were ionized with either EI or methane PCI using full scan MS or molecular-ion directed acquisition (MIDA -MS/MS.
The second, an "intelligent" data-dependent acquisition paradigm for small molecule discovery, termed "molecular-ion directed acquisition" or MIDA, maximizes the information content from data-dependent MS/MS and SIM by directing the instrument to sample the ions of greatest information content.
Additionally, stable-isotope labeling, in conjunction with our molecular-ion directed acquisition (MIDA) approach for MS/MS, guarantees not only information-rich MS/MS spectra for intact, or nearly intact, ionic species but also immediate readout of the number of carbon and nitrogen atoms present in each precursor and product ion species.
Second, with an "intelligent" data-dependent algorithm, termed molecular-ion directed acquisition (MIDA), we maximize the information content generated from unsupervised tandem MS (MS/MS) and selected ion monitoring (SIM) by directing the MS to target the ions of greatest information content, that is, the most-intact ionic species.
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Wu, M. F. et al. Auxin-regulated chromatin switch directs acquisition of flower primordium founder fate.
Thank you for submitting your work entitled "Auxin-regulated chromatin switch directs acquisition of flower primordium founder fate" for peer review at eLife.
By analyzing data and intelligently directing acquisition online, metadata generated at runtime may be used to improve the quality of information extracted from a data set.
At Yahoo, he was head of the team that directed the acquisition of Inktomi, AltaVista and FAST, in addition to helping to develop the company's search technology.
BHR participated in study design and directed data acquisition.
Drs. H. Boman, S. Johansson, and P. M. Knappskog directed the acquisition, analysis, and interpretation of the genetic data.
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