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For the biological effects of these resin composites on the HGF cells, the oral environmental factors mentioned earlier should be considered, and the application of direct viability test and also more than one surface characterization technique are essential for better understanding of the biological effects of resin composite brands to cells in proximal vicinity to them in the oral cavity.
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Direct PIR viability remained higher in hAMSC-treated hearts for >6 weeks.
These differences were noted despite careful efforts to exactly match inoculum sizes using direct plating viability counts.
After having superficially determined how the cell population responds to a photodynamic treatment (survival/proliferation, direct cytotoxicity/reduced viability), one may proceed to in-depth characterization of the specific mode of cell death in case of a cytotoxic PDT regimen.
Diluted blood reperfusion as a model for transplantation of ischemic rat livers: alanine aminotransferase is a direct indicator of viability.
Other studies have involved direct quantification and viability of parasite development by microscopy [26], [27], RNA hybridization [28], or infrared fluorescence scanning system [29].
To assess the effects of PM on A549 cell viability, direct cell counting was performed.
Importantly, none of these are direct markers of viability, and their kinetics may differ from that of bacterial culture.
First, we provide a direct comparison of viability among first- and second-generation hybrids to determine the strength of the initial barrier to gene exchange.
Caco-2 proliferation rate expressed by the cell viability (direct cell counting, measurement of total cell protein or DNA) or its enzymatic activity (MTT and WST-1 tests) often forms the basis for an assessment of cytotoxic potential of examined substances [ 14, 22, 28, 29, 55].
We conducted similar experiments with the D283med and D425med medulloblastoma cell lines to determine if the MV conditioned media had a direct impact on their viability as well, but we observed no variance in cell viability even with concentrations of conditioned media up to 500 μg/ml (data not shown).
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