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We designed two non-GUX hammerhead ribozymes (Rzs) and a Di-Rz by placing them in direct tandem.
HDP1 was a repetitive sequence with a 53 bp monomeric unit repeated 24 times in direct tandem along the 1272 bp fragment, while the 3954 bp HDP2 was not a repetitive sequence.
Direct tandem genetic fusion through restriction enzyme sites is simple; the flexible and unstructured N- or C-terminal regions of the component proteins and additional short peptides derived from restriction enzyme sites act as a peptide linker to provide enough space between the functional units of a fusion protein for correct folding.
The engine driving their model is the continuous genesis of adjacent direct (tandem) repeats created by mispairing and recombination between sister chromatids.
This longest copy is the only one with direct tandem repeats within the TIRs.
It is also compatible with mitotic and meiotic stability of interchromosomal duplications and direct tandem duplications [ 9, 43].
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The rISTs showed more than 99% identity with each other and were always organized in direct tandems between each copy of Hosim1.
The BP100 direct tandems did not show higher antimicrobial activity than BP100, and genetically modified (GM) plants constitutively expressing them were not viable.
Three of these tRNA pairs are organized in direct tandems with a short distance between the two genes: 2 × tDNA-Gly (GCC), 12 nt; 2 × tDNA-Asp (GTC), 29 nt; 2 × tDNA-Val (GAC), 45 nt probably revealing a recent gene duplication.
The writing and directing tandem of Larry and Andy Wachowski are as suited to the task of guiding this film as Neo is to saving the world from the onslaught of the Sentinels, computer emissaries bent on eliminating all vestiges of humanity.
Short direct repeats, tandem repeats and monotonic runs are known to promote strand misalignments during DNA replication and might explain the increased deletion frequency we observed in the H. salinarum NRC-1 pyrE2 gene [35].
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