Sentence examples for direct tagging from inspiring English sources

Therefore, the determinants responsible for tagging at these sites are not local sequence elements, because these sequences are not sufficient to direct tagging in other molecular contexts.

All transgenic and mutant parasite lines are derivatives of the RH parasite line: the RHTK+ strain expresses the fusion protein of herpes simplex thymidine kinase and chloramphenicol acetyltransferase [23]; temperature sensitive clone 88A5 was obtained by chemical mutagenesis of the RHΔhxgprt- strain [38]; RHΔku80 strain [51] was utilized for direct tagging of AP2 genes with YFP.

6 This is why gene tagging of transcriptome sequences is easier than direct tagging of genome sequences.

A potential problem with direct tagging is SH2 domain activity loss due to amino acid modification that would need to be assessed on a domain-by-domain basis.

These observations introduce the possibility of exploiting the antigen-tracing capabilities of chromobodies in intact organisms, while circumventing direct tagging or overexpression of the target protein, which are known causes of localization artefacts (Huh et al., 2003; Swulius and Jensen, 2012).

In summary, the present directed tagging method is facile and can yield productive cell lines from rare but precise recombination events.

Using cells from the LARC collection allows considerable shortening of the time required to generate double-labeled reporter lines, since only one round of directed tagging is required.

DNA in cancer cells is heavily tagged, even in CG-rich regions, and transcription factors do not appear to play a major role in directing tagging.

Following a directed tagging experiment (see materials and methods) five candidate pr1 kernels were identified from 37 test-cross ears (∼7400 kernels).

The intracellular expression of these affinity fluorescent probes in a range of cell types may permit the directed tagging of intracellular targets with fluorescent proteins for a range of molecular cell biology imaging studies currently not possible via direct fluorescent protein fusions.

Tagged smolts were generally held for ≥24 hr after surgery (up to several weeks for smolts tagged at hatcheries) to assess direct tagging-related mortality, tag extrusions, and monitor for signs of impaired swimming behaviour.