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In vitro and in vivo binding experiments demonstrated that Pin1 binds to N1-ICD and this interaction was direct (supplementary Fig S5B and C).
Moreover, by in silico analysis, using the TraFac Homology Server, we found the specific responsive elements of PATZ1 (here indicated as MAZR) in the promoter regions of MDM2 and CDKN1A, suggesting that the binding of PATZ1 to these genes may be direct (Supplementary Table S1).
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Two of the large gene deletions in this region were not associated with flanking direct repeats (supplementary table S2, Supplementary Material online).
The fraction of mutants having indels at the short tandem repeat and/or monitor loci is known for a finite number (64 109) of FUdR− + Cm-resistant colonies, as determined by selective plating and direct sequencing (supplementary table S3, Supplementary Material online).
These clusters are remarkably well in agreement with the lineages detected in the core genome phylogeny (fig. 1), and when the concatenated MLST phylogeny and resulting BAPS groupings for the whole-genome sequence strains is compared with the whole-genome phylogeny, there is direct concordance (supplementary fig. S1, Supplementary Material online).
PSA concentration and CTC count kinetics had no clear direct relationships (Supplementary Figure S1a, b ).
We found that Pn demonstrated little pyrophosphatase activity in a direct assay (Supplementary Fig S3A).
Therefore, the R108 ptDNA genome organization is derived, generated by an inversion via the short direct repeats (Supplementary Fig. S1).
After amplification using touchdown PCR, fragments were analysed through the Transgenomics WAVE DHPLC system followed by direct sequencing (Supplementary methods).
While all TAL1-regulated direct targets (Supplementary Table VI) are candidate effectors of TAL1-mediated leukaemogenesis, Cdk6, which is activated by TAL1, presents a particular interest.
Additionally, we could immunoprecipitate in vitro His-tagged Akirin prepared in bacteria with Flag-tagged Relish ΔS29 S45 purified from S2 cells suggesting their direct interaction (Supplementary Fig S7).
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