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Results of the bridged split YFP assay are generally consistent with the direct split YFP results, but appear more selective.
Some combinations of RH and TGA proteins appear to disrupt interactions observed in the direct split YFP assay between NH proteins and members of these other two families.
In direct split YFP assays, NH4 and NH5 interacted modestly with most rice TGA and RH proteins with few outstanding preferences.
NH2 did not show strong interaction with TGA proteins in the direct split YFP assay (Table 3), but does associate strongly with the six selected TGA proteins in the presence of ectopic RH2 or RH3 (Table 9).
For example, NH3 interacts well with RH3 and all six selected TGA proteins in the direct split YFP assay, but not in the bridged split YFP assay, where only rTGA2.3 and TGAL4 show a weak association with NH3 in the presence of ectopic RH3.
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This study demonstrates the optimized parameters of manufacturing HM OH 2 by direct splitting its halide for industrial application.
The scheme is based on the direct splitting of macroscopic flux functions with consideration of particle transport.
Direct splitting of seawater to produce H2 and O2 is also demonstrated using Rh2−yCryO3-loaded (Ga1−xZnx)(N1−xOx) catalyst under visible light.
The proposed numerical scheme is based on the direct splitting of macroscopic flux functions of the system of equations.
The new scheme is based on the direct splitting of the flux function of the MHD equations with the inclusion of "particle" collisions in the transport process.
The kinetic MHD scheme is constructed based on the direct splitting of the macroscopic flux functions with the consideration of particle transport.
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