Considering that this double mutant still contains the miR-122 complementary sequence in the 3′-UTR, the latter result confirms that direct base pairing between miRNA and the two miRNA target sites in the 5′-UTR of the HCV reporter RNA is involved in the stimulation of HCV translation.
Our experimental design involved direct pair-wise comparison between mutant and wild type at young age (3 months, 5 individuals per group) including a dye swap comparison (a total of 10 slides).
For the sake of the analysis presented here, we will focus on direct pair-wise comparisons between samples using spotted DNA arrays conducted as dual-labeled co-hybridization assays.
An E. coli motility expression data set ([ 41] series accession number: GPL2101) was obtained in a direct pair-wise comparison between a knock-out mutant of the flhDC, a master regulator of the motility/chemotaxis regulon [ 25], and its isogenic wild type strain.
To further support this statistical analysis, it was also tested if there is a significant difference between the direct pairs of A1 and B1, and A2 and B2, which would indicate the influence of gold NP alone.
Further, the partnership enables direct currency pairs between Japanese yen and the fiat currencies of Ghana, Kenya, Morocco, Nigeria, Senegal, Tanzania, Uganda and the Democratic Republic of Congo.
U1 snRNP recognizes the 5' splice site through direct base-pairing interactions between the pre-mRNA and the snRNA (55).
The key was to apply statistical methods that can distinguish direct couplings between pairs of columns in a multiple sequence alignment from merely correlated pairs, i.e. to separate direct from indirect effects.
He has adapted these tools for Spanish FrameNet and now Japanese FrameNet, allowing direct comparisons between pairs of languages.
To this end, recent proposals are focused on direct matching between pairs of features within sequences, mainly based on classical distances.
