Exact(1)
The sequence was incorporated into a plasmid containing the bacteriophage λ-integrase sites attB and attD in the direct orientation to allow disintegration of the MC upon transformation of strain LZ54.
Similar(59)
Recombination between DNA segments bordered by two repeated sequences positioned in direct orientation relative to each other can lead to duplication and subsequent amplification of the intervening DNA.
The majority of the repeated sequence in the genome is represented by a 1362 bp "core" repeat sequence that is present in 6 identical copies, all of which are in the same (i.e., direct) orientation relative to each other.
We noticed that the UAS promoter weakly affected wing and body pigmentation only when it was located in direct orientation relative to the yellow enhancers (Additional file 1: Figure S1).
All three test probes (TUPLE1, b186O8 and p901P22) were clearly triplicated, demonstrating a duplication in 22q11.2 with a direct orientation, corresponding to the longer (3 Mb) of the two recurrent CNVs observed in this region [ 26].
The internal components of the GBT-B1 vector (the gene trap cassette and the UAS:eGFP cassette) are flanked by loxP sites in direct orientation, analogously to the arrangement in GBT-R15 and GBT-RP2 [ 27, 28].
The eye enhancer ("e") flanked by frt sites was inserted in direct orientation relative to the white gene in the genomic position between the wing and body enhancers of the yellow gene.
All three regions carrying the CTTA units feature sequences that are in direct orientation relative to one another; however, the 13 regions with the GTAT and complementary ATAC units and the four regions with the TAGAA and complementary TTCTA units form a population of dispersed repeats that are in direct or inverted orientation relative to one another.
While the majority of individuals inherit this region in the direct orientation, up to 25% of individuals of European-Caucasian descent have an ∼970 Kb sequence in the opposite orientation (9, 12), inducing a larger ∼1.3 1.6 Mb region of linkage disequilibrium (LD) (13).
In pluripotent mouse TG-2a cells, pDEH6 plasmid, containing promoter and both enhancers (-2186/+106) in a direct orientation with respect to luciferase gene, displayed the highest luciferase activity, which exceeded the background level (compare to luciferase activity of empty vector pGL2-Basic) approximately six-fold.
Madia sativa IGS contained a highly mutated (52% homology) 5S sequence ~ 350 bp downstream from the 26S gene, a peculiar feature of this presumed 5S insertion is its direct orientation with respect to the 26S gene.
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