The last two types were joining of two segments in a direct orientation, accounting for 15% of the total chimeras.
This assumption is tested by a direct orientation measurement using Electron Back Scattering Patterns (EBSP).
Figure 11 Schematic representation of the direct orientation of radicals that are formed from the decomposition of α,α'-azoisobutyronitrile (AIBN) under microwave irradiation[47].
Recombination between DNA segments bordered by two repeated sequences positioned in direct orientation relative to each other can lead to duplication and subsequent amplification of the intervening DNA.
The two parts are homologous over at least several hundred base pairs and are arranged in either inverted or direct orientation.
In the present case, because each 14.6 kb insertion is composed of 5.3 kb terminal IRs, inversion could be the outcome even if the full-length structures were present in direct orientation, via pairing of opposite IR arms.
These transposon systems have two-components, the first of which is a transposon vector containing an expression cassette flanked by terminal inverted repeats that have binding sites for the SB or PB transposase in direct orientation, termed IR/DRs.
In the first cell line (C'10) the two I-Sce-I sites are in direct orientation resulting in CD4 expression after deletion events (the most frequent events) or CD8 expression after invertion events.
Two hexamers (subsites I and III) are in the direct orientation and one (subsite II) is in the inverse orientation (→4-bp←4-bp→), conforming to a typical Fur box [33].
If the backbone of pDonor with loxP was excised, it was necessary to add another loxP, as a selectable marker gene should be placed between two loxP sites in direct orientation.
means direct orientation; - reverse orientation of the AuxREP&S elements.
